THE VALUE OF IMMUNOHISTOCHEMISTRY
IN THE ASSESSMENT OF BONE MARROW DISORDERS
Attilio Orazi, M.D., FRCPath. and Dennis P. O'Malley, M.D.
Several commercially available antibodies can be used to stain human p53 protein. The paraffin reactive
antibodies (e.g. Pab1801, DO7) recognize both wild and mutant forms of p53 protein. Both reagents works
well in formalin and B5 fixed tissues but requires heat-induced epitope retrieval. P53 overexpression is
present in acute leukemias, particularly in those secondary to chronic myeloproliferative diseases and
myelodysplastic syndromes, and in therapy-related cases. Reactivity can also be seen in high grade
lymphomas, and in various metastatic carcinomas.
- Brynes et al: Expression of p53, MDM2, p21waf1, bcl-2, and retinoblastoma gene proteins in myelodysplastic
syndrome after autologous bone marrow transplantation for lymphoma. Mod Pathol. 1997 Nov;10(11):1120-7.
- Elghetany et al: Significance of p53 overexpression in bone marrow biopsies from patients with bone marrow
failure: aplastic anemia, hypocellular refractory anemia, and hypercellular refractory anemia. Ann
Hematol. 1998 Dec;77(6):261-4.
- Kanavaros et al: Immunohistochemical detection of p53, mdm2, waf1/p21, and Ki67 proteins in bone marrow
biopsies in myelodysplastic syndroms, acute myelogenous leukaemias and chronic myeloproliferative disorders.
Clin Exp Pathol. 1999;47:231-8.
- Magalhaes et al: p53 overexpression in refractory anemia. An immunohistochemical analysis of bone marrow
biopsies. Haematologica. 1999; 84:377-8.
- Orazi A, Cattoretti G, Heerema NA, Sozzi G, John K, Neiman RS: Frequent p53 overexpression in therapy
related myelodysplastic syndromes and acute myeloid leukemias: an immunohistochemical study of bone marrow
biopsies. Mod Pathol 1993;6:521-525.