THE VALUE OF IMMUNOHISTOCHEMISTRY
IN THE ASSESSMENT OF BONE MARROW DISORDERS
Attilio Orazi, M.D., FRCPath. and Dennis P. O'Malley, M.D.
Anti-CD30 is a mouse monoclonal antibody made against cultured cell lines of CD30 positive cells. The
antigen is expressed by Hodgkin's and Reed-Sternberg cells, by the cells of anaplastic large cell lymphomas
and by a varying number of activated B and T cells. We use a heat induced epitope retrieval technique and
find that the stain works well in formalin fixed and B5-fixed tissue and works well in decalcified material.
It is important to recognize that CD30 is not a unique stain for anaplastic large cell lymphoma, for it is
characteristically positive in many peripheral T cell and B cell lymphomas. It also stains lymphoid cells
which harbor Epstein-Barr virus. Its primary use in bone marrow is to detect anaplastic large cell lymphoma
or Hodgkin's disease. CD30 also stains the cells of embryonal cell carcinoma and other non hematopoietic
tumors, melanoma in particular.
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cytokine receptor of the tumor necrosis factor receptor superfamily as a tool for diagnosis and
immunotherapy. Blood 1995;85:1.
- Pallesen G, Hamilton-Dutoiit SJ. Ki-1 (CD30) antigen is regularly expressed by tumor cells of embryonal
carcinoma. Am J Pathol 1988;133:446.
- Schwab U, Stein H, Gerdes J, Lemke H, Kirchner H, Schaadt M, et al. Production of a monoclonal antibody
specific for Hodgkin and Sternberg-Reed cells of Hodgkin's disease and a subset of normal lymphoid cells.
- Schwarting R, Gerdes J, Durkop H, Falini B, Pileri S, Stein H. Ber-H2: a new anti-K1-1 (CD30)
monoclonal antibody directed at a formol-resistant epitope. Blood 1989;74:1678.
- Stein H, Mason DY, Gerdes J, O'Connor N, Wainscoat J, Pallesen G, et al. The expression of the
Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue: evidence that
Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood