BCL-2 is a monoclonal antibody developed in mice to the human BCL-2 oncoprotein. It is most effective
demonstrated by heat-induced epitope retrieval techniques. It works well in either formalin or B5 fixed
tissues and is resistant to acid decalcification. In our hands it appears to work better in B5 than in
The BCL2 stain can be used to distinguish follicular lymphocytes from reactive follicular hyperplasia in
bone marrow. However, the effective value of this approach is still controversial (Fakan et al, 1996).
- Ben-Ezra JM et al. Staining for Bcl-2 protein helps to distinguish benign from malignant lymphoid
aggregates in bone marrow biopsies. Mod Pathol. 1994 ;7:560-4.
- Chetty et al: Immunohistochemistry in apparently normal bone marrow trephine specimens from patients
with nodal follicular lymphoma. J Clin Pathol. 1995; 48:1035-8.
- Fakan et al: Expression of bcl-2 protein in distinguishing benign from malignant lymphoid aggregates in
bone marrow biopsies. Gen Diagn Pathol. 1996;141:359-63.